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Publications
- Broom, A., Doxey, A.C., Lobsanov, Y.D., Berthin, L.G., Rose, D.R., Howell, P.L., McConkey, B.J.*, and Meiering, E.M.* Modular Evolution and the Origins of Symmetry: Reconstruction of a Three-Fold Symmetric Globular Protein. Structure 20, 161-171 (2012).
- Vassal, K.A., Stubbs, H.R., Primmer, H.A., Tong, M.S., Sullivan, S.M., Sobering, R., Srinivasan, S., Briere, L.A.K., Dunn, S.D., Colon, W., and Meiering, E.M.* Decreased stability and increased formation of soluble aggregates by immature superoxide dismutase do not account for disease severity in ALS. PNAS 108, 2210-2215 (2011).
- Smith, M.T.J., MacKenzie, D.W.S., and Meiering, E.M.* Dissecting the molecular determinants of ligand-binding-induced macromolecular switching using thermodynamic cycles. PEDS 24, 213-217 (2011).
- Hwang, Y.M., Stathopulos, P.B., Dimmick, K., Yang, H., Badiei, H.R., Tong, M.S., Rumfeldt, J.A.O., Chen, P., Karanassios, V., and Meiering, E.M.* Nonamyloid Aggregates Arising from Mature Copper/Zinc Superoxide Dismutases Resemble Those Observed in Amyotrophic Lateral Sclerosis. JBC 285, 41701-41711 (2010).
- Smith, M.T.J., Meissner, J., Esmonde, S., Wong, H.J., and Meiering, E.M.* Energetics and mechanmisms of folding and flipping the myristoyl switch in the beta-trefoil protein, hisactophilin. PNAS 107, 20952-20957 (2010).
- Galvagnion, C., Meglei, G., Vassall, K.A., Gaspar, J.A.,
Stathopulos, P.B., Cheyne, B. and Meiering, E.M.* Folding and
association of thermophilic dimeric and trimeric DsrEFH proteins:
Tm0979 and Mth1491. Biochemistry 48, 2891-2906 (2009).
- Rumfeldt, J.A.O., Lepock, J.R. and
Meiering, E.M.* Unfolding and folding kinetics of amyotrophic lateral
sclerosis-associated mutant Cu,Zn superoxide dismutases. J. Mol. Biol. 385, 278-98 (2009).
- Doxey, A.C., Lynch, M.D.J., Muller, K.M., Meiering, E.M. and
McConkey, B.J.* Evolutionary origins of clostridial neurotoxins. BMC Evolutionary Biology 8, 316 (9 pages) (2008).
- Meiering, E.M.* The threat of instability: neurodegeneration
predicted by protein destabilization and aggregation propensity. PLoS Biol. 6, e193 (2008).
- Rumfeldt, J.A.O., Galvagnion, C., Vassall, K.A. and
Meiering, E.M.* Conformational stability and folding mechanisms of dimeric proteins. Prog. Biophys. Mol. Biol. 98, 61-84 (2008).
- Vassall, K.A., Stathopulos, P.B., Rumfeldt, J.A.O., Lepock, J.R.
and Meiering, E.M.* Equilibrium thermodynamic analysis of amyotrophic
lateral sclerosis-associated mutant apo Cu,Zn superoxide
dismutases. Biochemistry 45, 7366-79 (2006).
- Stathopulos, P.B., Rumfeldt, J.A.O., Siddall, C.A.,
Lepock, J.R., and Meiering, E.M.* Calorimetric analysis of
thermodynamic stability and aggregation for apo and holo ALS-associated
gly93 mutants of superoxide dismutase. Journal of Biological Chemistry 281, 6184-93 (2006).
- Rumfeldt, J.A.O., Stathopulos, P.B., Chakrabartty, A.,
Lepock, J.R. and Meiering, E.M.* Mechanism and thermodynamics of
guanidinium chloride-induced denaturation of ALS-associated mutant
Cu,Zn superoxide dismutases. Journal of Molecular Biology 355, 106-123 (2006).
- Gaspar, J.A., Liu, C., Vassall, K.A., Meglei, G., Stephen,
R., Stathopulos, P.B., Pineda-Lucena, A., Wu, B., Yee, A., Arrowsmith,
C.H. and Meiering, E.M.* A novel member of the YchN-like fold: solution
structure of the hypothetical protein Tm0979 form Thermotoga maritima. Protein Science 14, 216-223 (2005).
- Wong, H.J., Stathopulos, P.B., Bonner, J.M., Sawyer, M. and
Meiering, E.M.* Nonlinear Effects of Temperature and Urea on the
Thermodynamics and Kinetics of Folding and Unfolding of
Hisactophilin. Journal of Molecular Biology 344, 1089-1107 (2004).
- Stathopulos, P.B., Scholz, G.A., Rumfeldt, J.A.O., Lepock,
J.R. and Meiering, E.M.* Sonication of proteins causes formation of
aggregates that resemble amyloid. Protein Science 13, 3017-3027 (2004).
- Stathopulos, P.B., Rumfeldt, J.A.O., Scholz, G.A., Irani,
R.A., Frey, H.E., Hallewell, R.A., Lepock, J.R. and Meiering, E.M.*
Cu/Zn superoxide dismutase mutants associated with amyotrophic lateral
sclerosis show enhanced formation of aggregates in vitro. PNAS USA 100, 7021-7026 (2003).
- Liu, C., Gaspar, J.A., Wong, H.J. and Meiering,
E.M.*Conserved and nonconserved features of the folding pathway of
hisactophilin, a b-trefoil protein. Protein Science 11, 669-679 (2002).
- Houliston, R.S., Liu, C., Singh, L.M.R. and Meiering, E.M.*
pH and urea dependence of amide hydrogen-deuterium exchange rates in
b-trefoil protein hisactophilin. Biochemistry 41, 1182-94 (2002).
- Liu, C., Chu, D., Wideman, R.D., Houliston, R.S., Wong,
H.J. and Meiering, E.M.* Thermodynamics of denaturation of
hisactophilin, a b-trefoil protein. Biochemistry 40, 3817-27 (2001).
- Hammond, M.S., Houliston, R.S. and Meiering, E.M.* Two-dimensional 1H and 15N NMR titration studies of hisactophilin. Biochem. Cell Biol. 76, 294-301 (1998).
- Wilquet, V., Gaspar, J.A., van de Lande, M., van de
Casteele, M., Legrain, C., Meiering, E.M. and Glandsorff, N.*
Purification and characterization of recombinant Thermotoga maritima dihydrofolate reductase. Eur. J. Biochem. 255, 628-637 (1998).
- Johnson, J.M., Meiering, E.M., Wright, J.E., Pardo, J.,
Rosowsky, A. and Wagner, G.* NMR solution structure of the
antitumour compound PT523 and NADPH in the ternary complex with human
dihydrofolate reductase. Biochemistry 36, 4399-4411 (1997).
- Meiering, E.M., Li, H., Delcamp, T.J., Freisheim, J.H. and
Wagner, G.* Contributions of tryptophan-24 and glutamate-30 to binding
long-lived bound water molecules in the ternary complex of human
dihydrofolate reductase with methotrexate and NADPH studied by
site-directed mutagenesis and NMR. J. Mol. Biol. 247, 309-325 (1995).
- Meiering, E.M. and Wagner, G.* Detection of
long-lived bound water molecules in complexes of human dihydrofolate
reductase with methotrexate and NADPH. J. Mol. Biol. 247, 294-308 (1995).
- Anteneodo, C., Rodahl, A.M., Meiering, E., Heynen, M.L.,
Sennisterra, G.A. and Lepock, J.R.* Interaction of dibucaine with the
transmembrane domain of the Ca2+-ATPase of sarcoplasmic
reticulum. Biochemistry 33, 12283-12290 (1994).
- Meiering, E.M., Bycroft, M. and Fersht, A.R.* The structure
and dynamics of barnase complexed with 3’GMP studied by NMR
spectroscopy. Biochemistry 32, 10975-10987 (1993).
- Meiering, E.M., Serrano, L. and Fersht, A.R.* Effect of active site residues in barnase on stability and activity. J. Mol. Biol. 225, 585-589 (1992).
- Matouschek, A., Serrano, L., Meiering, E.M., Bycroft, M.
and Fersht, A.R.* The folding of an enzyme V. H/D exchange-NMR
studies on the folding pathway of barnase: complementarity to and
agreement with protein engineering studies. J. Mol. Biol. 224, 837-845 (1992).
- Meiering, E.M., Bycroft, M., and Fersht, A.R.*
Characterization of phosphate binding to the ribonuclease barnase by
site-directed mutagenesis and NMR. Biochemistry 30, 11348-11356 (1991).
- Sancho, J., Meiering, E.M., and A.R. Fersht* Mapping
transition states of protein folding by protein engineering of ligand
binding sites. J. Mol. Biol. 221, 1007-1014 (1991).
- Anderson, A.*, Andrews, B., Meiering, E.M. and Torrie, B.H.
Raman and far-infrared study of the lattice vibrations of
methanol. J. Raman Spectrosc. 19, 85-91 (1988).
